The humanized mouse (hu-mouse) model has revolutionized the ability to carry out in vivo studies of the human immune system.
With over 25 years of experience developing hu-mouse models with functional human hematopoietic and lymphoid systems, we have pioneered novel humanized mouse models with robust, functional human immune systems. Our original model has been replicated in many other laboratories (sometimes referred to as the “BLT” mouse). The application of this model has been pivotal in the exploration of human immune responses and disorders. Our expertise in this field was conducive to the development of other models, one of which gained renown as the Personalized Immune (PI) humanized mouse with the transplantation of patient-specific hematopoietic stem cells (HSCs). This “personalized immune” mouse provides a new model for individualized analysis of human HSC-intrinsic immune abnormalities predisposing to autoimmunity as well as model individual human immune responses in both health and diseases. Furthermore, we established the Hu-mouse model with Patient-derived-xenograft (PDX) tumors, in which the infiltration of human immune cells in the tumor microenvironment leads this model to be an indispensable tool to determine patient eligibility for novel immunotherapies, treatment options, and mechanisms of immune surveillance in solid tumors. Additionally, we have established protocols for humanized mice with human T cells that express tumor or viral antigen-specific TCRs as well as humanized mice that spontaneously develop autologous human leukemia by transplanting genetically manipulated HSCs. These refinements further expand the potential applications of the hu-mouse model. By providing our services to other laboratories, we aspire to support investigation of human immunology and expand our repertoire of hu-mouse models.
Core Services include breeding and maintenance of NSG (NOD.Cg-Prkdcscid Il2rgtm1Wjl/SzJ) mouse colonies, construction of humanized mice, as well as confirmation of reconstitution of human hematopoietic and lymphoid systems by detection of >25% human cells (including T, B, and myeloid cells) in peripheral blood 8-10 weeks post-engraftment.
Standard Hu-Mouse (Functional Human Immune System)
- Core provides standard hu-mice engrafted with human fetal Thymus + HSCs or HSCs only;
- Core constructs hu-mice with specific transgenic backgrounds (HLA-A2, HLA-DQ8, hSGM3, etc.) to suit investigator’s needs;
- Core can occasionally provide a few (<10) standard hu-mice for pilot express studies but we DO NOT regularly stockpile standard hu-mice.
Personalized Immune (PI) Hu-Mouse (Patient-specific Human Immune System)
- Investigators provide patient HLA-typed specimens (CD34+ cells from bone marrow or mobilized PBMC);
- Core provides HLA-typed thymic tissue and constructs PI mice.
Patient-Derived Xenografts (PDX)
- Investigators provide xenografts or cells from patient tumor samples;
- Core constructs PDX model;
- Core propagates tumor in mice and cryopreserved tumor for future use upon request.
PDX/Standard or /PI Hu-Mouse
- Core engrafts PDX into pre-established partial HLA-matched allogeneic Standard or autologous PI Hu-Mouse;
- Core evaluates in vivo efficacy of immunotherapy agents either alone or in combination with standard chemotherapy regimens against human tumors, for an extra charge.
Customized Hu-Mouse (Compromised Human Immune System)
- Core provides hu-mice engrafted with virally transduced HSCs. Investigators are encouraged to provide their own transduced HSCs or viral vectors.
Rates per mouse
(for cohorts of at least 5 mice; a labor surcharge will apply to smaller cohorts)
Standard Hu-Mouse (Thymus+HSC's)
- Internal Cost: $450
- External Cost: $750
Hu-Mouse (HSC's Only)
- Internal Cost: $410
- External Cost: $680
Personalized Immune Hu-Mouse
- Internal Cost: $500
- External Cost: $830
Patient-Derived Xenografts (PDX)
- Internal Cost: $350
- External Cost: $600
- Internal Cost: $700
- External Cost: $1,200
- Internal Cost: $750
- External Cost: $1,500
- Internal Cost: TBD
- External Cost: TBD
Additional Housing: $0.84/hu-mouse/day
Please submit the Humanized Mouse Request Form at least 2 months prior to intended start date of hu-mouse reconstitution. Once hu-mice reconstitution has been confirmed (8-10 weeks), hu-mice are transferred to investigator’s mouse facility, unless Humanized Mouse Core (HMC) housing is required.
It is advised that interested investigators contact us before submission of the request form to thoroughly discuss project and options.
We can not start to construct hu-mice without chartstring information or purchase order provided by internal or external customers, respectively.
If you feature this work in future publications, please acknowledge as much in your paper with the following statement:
“Humanized mouse was provided by Humanized Mouse Core (HMC) Facility, CCTI, CUMC, Columbia University”
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Megan Sykes, MD