Protocols
Mouse/Atherosclerosis/Metabolism Protocols
- NASH mouse model based on FPC-NASH diet
- Tips on Mouse Handling
- Assay of Hepatic p-CaMKII in Fasting Mice
- Liver Macrophages Isolation
- Genotyping of Asm-/- Mice
- Protocol for Bone Marrow Transplantation
- Principles of Mouse Husbandry: George Kuriakose's 2013 PowerPoint Presentation
- Assessment of Progression of Atherosclerotic Lesions in Mice
- Mouse Shipping Information
- Genotyping of ACAT-/-Mice
- Genotyping of F4/80-Cre Knock-In Mice
- Basic Information for Directing a Mouse Atherosclerosis Project
- Important Information About the Harlan-Teklad 88137 Western Diet Used in the Tabas Laboratory for Mouse Atherosclerosis Studies
- More Detailed Procedure for Embedding Mouse Hearts in OCT in Preparation for Frozen Sections
- Shipment of Mouse Femurs for Use in Isolating Marrow Cells for In Vitro MΦ Studies or BMT
- Fundamental Principles of Conducting Mouse Obesity Studies
- P-Akt, p-HSP27, and p-MK2 Assays in Obese Mice
- DIO vs. ob/ob
- Dex-thymus
Lipoprotein Protocols
- Protocol for Reconstitution of the Hydrophobic Core of Acetyl-LDL
- Labeling of Acetyl-LDL with DiI (1,1'-dioetadeeyl-3,3,3',3'-tetramethylindocarboeyanine perchlorate)
- Protocol for Preparation of LDL from Human Plasma
- Precautions to avoid endotoxin contamination of LDL and Acetyl-LDL
Cell Protocols
- L Cell Conditioned Medium (LCCM)
- Preparation of Adherent Macrophages for Electron Microscopy
- Technical Points About Plasmid-Mediated Transfection or RNAi-Mediated Gene Knockdown in Methyl-BSA-Elicited Macrophages Using the Amaxa Nucleofector® System (Dr. Yankun Li, September, 2005)
- Concanavalin A-Elicited Mouse Peritoneal Macrophages
- Preparation of Peritoneal Macrophages from Methylated BSA-Immunized Mice
- Filipin Fluorescence Staining of Free Cholesterol in Cultured Cells
- Isolation of bone marrow macrophage
- Isolating Cytosolic and Mitochondrial Extracts from Mouse Macrophages
- Isolating Smooth Muscle Cells from Mouse Aorta
- Preparation of Apoptotic Jurkat Cells for Efferocytosis Assays
- Protocol – Flow cytometry analysis of IL-10 and TGF-ß
- PMN isolation protocol
- Seahorse XF96 Protocol – adapted from Agilent
- Human macrophage isolation
Assays
- Standardized Tabas Laboratory In-Vitro Efferocytosis Engulfment Assay
- Immunoprecipitations Using TrueBlot
- GC Assay for Cellular Cholesterol Mass
- CHOP Immunoblots of Whole Cell Extracts
- Preparation of 14 C-Oleate-Albumin Solution for Whole-Cell Cholesterol Esterification Assay
- Method for Preparing Cytoplasmic and Nuclear Extracts for Immunoblots of UPR Proteins
- Assay for Apoptosis of FC-Loaded Macrophages (Annexin/propidium iodide kit is from Molecular Probes)
- Extraction of Lipids in Solution by the Method of Bligh & Dyer
- Euglobulin Clot Lysis Assay for Mouse Plasma